Chu 13

CHU 13 medium is a culture medium used in microbiology for the growth of certain algal species, first published by Chu SP in 1942. [1] It is used as growth medium for biofuel candidate alga Botryococcus braunii . [2] CHU 13 includes essential minerals and trace elements that are required for growth, but does not include a carbon source and is only appropriate for growth of phototrophs . It can be prepared as a medium or medium agar medium.

Modified CHU13 Medium, one liter [3]
Compound mg / L
KNO 3 400
2 HPO 4 80
CaCl 2 dihydrate 107
MgSO 4 heptahydrate 200
Ferric Citrate 20
Citric acid 100
CoCl 2 0.02
3 BO 3 5.72
MnCl 2 tetrahydrate 3.62
ZnSO 4 heptahydrate 0.44
CuSO 4 pentahydrate 0.16
Na 2 MoO 4 0084
0.072 N H 2 SO 4 1 drop

The remaining volume is pure, de-ionized water.

Because it is difficult to weigh some of the trace minerals, it is advisable to create a mixture of all components at a large concentration, such as a thousand times these measures, and then mix with the appropriate amount of (pure, de-ionized ) water. Correct pH to 7.5, and then autoclave.

References

  1. Jump up^ SP Chu (Aug. 1942) The Journal of Ecology, Vol. 30, No. 2, pp. 284-325.
  2. Jump up^ C. Largeau E. Casadevall, C. and P. Berkaloff Dhamelincout (1980) Phytochemistry, 19, 1943.
  3. Jump up^ K. Yamaguchi; et al. (1987). “Lipid composition of a green alga, Botryococcus braunii”. Agric. Biol. Chem . 51 (2): 493-498. doi : 10.1271 / bbb1961.51.493 .

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