CHU 13 medium is a culture medium used in microbiology for the growth of certain algal species, first published by Chu SP in 1942.  It is used as growth medium for biofuel candidate alga Botryococcus braunii .  CHU 13 includes essential minerals and trace elements that are required for growth, but does not include a carbon source and is only appropriate for growth of phototrophs . It can be prepared as a medium or medium agar medium.
|Compound||mg / L|
|K 2 HPO 4||80|
|CaCl 2 dihydrate||107|
|MgSO 4 heptahydrate||200|
|H 3 BO 3||5.72|
|MnCl 2 tetrahydrate||3.62|
|ZnSO 4 heptahydrate||0.44|
|CuSO 4 pentahydrate||0.16|
|Na 2 MoO 4||0084|
|0.072 N H 2 SO 4||1 drop|
The remaining volume is pure, de-ionized water.
Because it is difficult to weigh some of the trace minerals, it is advisable to create a mixture of all components at a large concentration, such as a thousand times these measures, and then mix with the appropriate amount of (pure, de-ionized ) water. Correct pH to 7.5, and then autoclave.
- Jump up^ SP Chu (Aug. 1942) The Journal of Ecology, Vol. 30, No. 2, pp. 284-325.
- Jump up^ C. Largeau E. Casadevall, C. and P. Berkaloff Dhamelincout (1980) Phytochemistry, 19, 1943.
- Jump up^ K. Yamaguchi; et al. (1987). “Lipid composition of a green alga, Botryococcus braunii”. Agric. Biol. Chem . 51 (2): 493-498. doi : 10.1271 / bbb1961.51.493 .